Mastercycler^® X50

The new Mastercycler X50 is the elegant synthesis of speed, flexibility and PCR optimization for research PCR applications and standardization for routine PCR applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. More information

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Mastercycler^® X50s, 110 – 230 V/50 – 60 Hz, silver block, 96-well plate or 0.1/0.2 mL tubes, with touch screen interface Catalog No. 6311000010

Net Price

9.135,00 €

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9.135,00 €

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Mastercycler^® X50a, 110 – 230 V/50 – 60 Hz, aluminum block, 96-well plate or 0.1/0.2 mL tubes, with touch screen interface Catalog No. 6313000018

Net Price

8.628,00 €

Your Price

8.628,00 €

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Mastercycler^® X50p, 110 – 230 V/50 – 60 Hz, aluminum block, 96-well plate, high pressure lid, with touch screen interface Catalog No. 6315000015

Net Price

9.135,00 €

Your Price

9.135,00 €

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Mastercycler^® X50h, 110 – 230 V/50 – 60 Hz, aluminum block, 384-well plate, high pressure lid, with touch screen interface Catalog No. 6316000019

Net Price

9.135,00 €

Your Price

9.135,00 €

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Mastercycler^® X50i, 110 – 230 V/50 – 60 Hz, silver block, 96-well plate or 0.1/0.2 mL tubes, eco unit Catalog No. 6301000012

Net Price

7.511,00 €

Your Price

7.511,00 €

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Mastercycler^® X50l, 110 – 230 V/50 – 60 Hz, aluminum block, 96-well plate or 0.1/0.2 mL tubes, eco unit Catalog No. 6303000010

Net Price

7.004,00 €

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7.004,00 €

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Mastercycler^® X50r, 110 – 230 V/50 – 60 Hz, aluminum block, 96-well plate, high pressure lid, eco unit Catalog No. 6305000017

Net Price

7.511,00 €

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7.511,00 €

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Mastercycler^® X50t, 110 – 230 V/50 – 60 Hz, aluminum block, 384-well plate, high pressure lid, eco unit Catalog No. 6306000010

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8.019,00 €

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8.019,00 €

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Product Information

The Mastercycler X50 family boasts ramp rates of up to 10°C/s and is the ideal cycler family for routine applications as well as advanced research in molecular biology.

The Mastercycler X50 family consist of units with a user-friendly touchscreen interface or eco units which are externally controlled. Each cycler with a touchscreen can be networked with nine additional eco units. If that is not enough throughput, you can network up fifty Mastercycler eco units to a computer or laptop running CycleManager X50 as a central controller. Whichever you choose, you can independently control and monitor every unit. You can also manage who has access to the system and designate a level of access with our intuitive user interface. Low noise levels, low power consumption, and the versatile flexlid^® concept complete the product to be a powerful and convenient PCR cycler.

PCR Optimization of β-actin Gene with 2D-Gradient technique.

2D-Gradient allows you to optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) during the same run. Higher denaturation temperatures can have the advantage of increased specificity while lower denaturation temperatures reduce stress on biomolecules and can lead to increased yield. Assays that struggle to work reliably at a 95° denaturation temperature could benefit significantly from the optimization of the denaturation temperature.

PCR should be faster. That gives you quicker results, throughout the day, and a faster transition to optimal temperatures. The Mastercycler X50 heats with 10 °C/s and cools with 5 °C/s. Make the speed of the Mastercycler X50 your research advantage.

There ia a large variety of cyclers available with different ramp rates published.
If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not. For more details, see Application Note 274.

Features

Heating rate: up to 10 °C/s
Wide selection of blocks from a fast silver block to 384 well block
Touch screen interface
Innovative 2D-Gradient for advanced PCR optimization
X50 with Touchscreen can control up to 9 other units (connected cyclers do not have a touch screen interface)
flexlid^® concept: automatic height adjustment of the lid allows you to use all types of consumables
Small footprint
2 Year warranty
Warranty: 2 Year warranty

Applications

Fast PCR
PCR optimization
Standard PCR
Incubation
Cycle sequencing

	MC X50s	MC X50a	MC X50p	MC X50h	X50i	X50l	X50r	X50t
For use with (application)	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing	Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing
Touchscreen Interface	￭	￭	￭	￭	–	–	–	–
Unit can control 9 other units in network	￭	￭	￭	￭	–	–	–	–
Unit can only be controlled	–	–	–	–	￭	￭	￭	￭
Thermoblock	Silver	Aluminum	Aluminum	Aluminum	Silver	Aluminum	Aluminum	Aluminum
Sample capacity	96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)	96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)	1 PCR plate (96 wells)	1 PCR plate (384 wells)	96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)	96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)	1 PCR plate (96 wells)	1 PCR plate (384 wells)
96-well plate	￭	￭	￭	–	￭	￭	￭	–
384-well plate	–	–	–	￭	–	–	–	￭
0.1/0.2 mL tubes	￭	￭	–	–	￭	￭	–	–
Heating technology	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements
2D-Gradient block	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 24 columns/over 16 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 24 columns/over 16 rows
Temperature control mode	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe
Temperature control range of the block	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C
Gradient range	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C
Gradient temperature range	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C
Block homogeneity 20–72 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C
Block homogeneity 95 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C
Control accuracy	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C
Heating rate	10 °C/s	5 °C/s	5 °C/s	5 °C/s	10 °C/s	5 °C/s	5 °C/s	5 °C/s
Cooling rate	5 °C/s	2.3 °C/s	2.3 °C/s	2.3 °C/s	5 °C/s	2.3 °C/s	2.3 °C/s	2.3 °C/s
Lid descent	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP
High pressure lid	–	–	￭	￭	–	–	￭	￭
Lid temperature range	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C
Interfaces	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB
Power supply	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz
Max. power consumption	850 W	850 W	850 W	850 W	850 W	850 W	850 W	850 W
Total weight	11.5 kg	11.5 kg	11.5 kg	11.5 kg	10.7 kg	10.7 kg	10.7 kg	10.7 kg
Dimensions (W × D × H)	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm	27,5 × 43 × 33 cm